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title: Protocols & Troubleshooting Guides | Resource Center
description: Protocols & Troubleshooting Guides
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1. [Resource Center](https://knowledge.kactusbio.com/)
2. [Protocols & Troubleshooting Guides](https://knowledge.kactusbio.com/protocols-troubleshooting-guides)

# Protocols & Troubleshooting Guides

- [How to determine RNA Yield](https://knowledge.kactusbio.com/determine-rna-yield)
- [How to detect duplex dsRNA (double stranded RNA)](https://knowledge.kactusbio.com/how-to-detect)
- [How to evaluate T7 RNAP processivity (Long RNA transcripts)](https://knowledge.kactusbio.com/how-to-evaluate-t7-rnap-processivity-long-rna-transcripts)
- [How to quantify dsRNA in IVT samples using MDA5-ATPase assay](https://knowledge.kactusbio.com/how-to-quantify)
- [What is the CircRNA (Circular RNA) Group I Method 1?](https://knowledge.kactusbio.com/what-is-the-circrna-circular-rna-group-i-method1)
- [What’s the DNA and RNA 3’- extension protocol and how to detect it with Urea-PAGE (Sequencing gel) (Ref 1)?](https://knowledge.kactusbio.com/whats-the-dna-and-rna-3-extension-protocol-and-how-to-detect-it-with-urea-page-sequencing-gel-ref-1)
- [Tetrameric MHC staining protocol for flow cytometry](https://knowledge.kactusbio.com/tetrameric-mhc-staining-protocol-for-flow-cytometry)
- [ELISA Troubleshooting Guide](https://knowledge.kactusbio.com/elisa-troubleshooting-guide)
- [Surface Plasmon Resonance (SPR) Troubleshooting Guide](https://knowledge.kactusbio.com/trouble-shooting-guide-for-surface-plasmon-resonance-spr)
- [Laminin 521: Recommended Protocol](https://knowledge.kactusbio.com/laminin-521-recommended-protocol)

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